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. 2025 Mar 14;17(3):699–725. doi: 10.18632/aging.206221

Table 6. Top 20 DNA methylation regions significant differences between 12 months following supplementation and baseline.

Chr Start position End position Width Number of CpG sites Overlapping genes Mean differential Min smoothed FDR HMFDR
chr5 179224563 179226186 1624 12 MGAT4B, MIR1229 0.0378 1.24E-39 5.21E-19
chr16 69140794 69141478 685 11 HAS3 −0.0063 1.73E-18 4.04E-12
chr17 43972306 43973522 1217 10 MAPT, MAPT-AS1 −0.0134 1.26E-22 5.53E-12
chr2 65659148 65660700 1553 12 SPRED2 −0.0151 2.12E-18 3.62E-11
chr16 56965376 56966475 1100 12 HERPUD1 −0.0041 2.63E-17 4.52E-11
chr22 19466331 19467326 996 15 CDC45, UFD1L −0.0059 5.39E-23 7.43E-11
chr10 1033582 1035006 1425 15 GTPBP4, AL359878.1 −0.0049 1.28E-14 9.76E-11
chr4 2964097 2965549 1453 15 GRK4, NOP14 −0.0105 4.96E-19 1.42E-10
chr11 105947676 105949106 1431 23 AASDHPPT, KBTBD3 −0.0053 1.03E-16 1.47E-10
chr4 75859212 75860346 1135 6 PARM1 −0.0858 8.28E-18 2.22E-10
chr7 75795555 75796528 974 7 −0.0152 6.79E-16 1.02E-09
chr3 15468722 15469386 665 12 EAF1, METTL6 −0.0113 9.94E-18 1.18E-09
chr16 11438416 11439785 1370 13 RMI2, RP11-485G7.5 −0.0106 3.38E-14 2.33E-09
chr12 58165256 58166652 1397 16 METTL21B, METTL1 −0.0096 5.30E-15 3.78E-09
chr2 180871638 180872164 527 14 CWC22 −0.0654 2.33E-30 5.67E-09
chr11 65728931 65729635 705 9 SART1 −0.0053 4.52E-11 1.19E-08
chr11 94822598 94823603 1006 6 ENDOD1 −0.0084 1.29E-18 1.44E-08
chr14 100070556 100071437 882 9 RP11-543C4.1 −0.0118 1.26E-12 2.79E-08
chr17 79633202 79633857 656 15 CCDC137, OXLD1 −0.0068 1.47E-09 3.64E-08
chr15 63333846 63335016 1171 8 TPM1 −0.0080 3.75E-15 3.89E-08

The regions are annotated by their chromosomal location (chr), their start and end base pair position, and the number of base pairs they span (width). The number of differentially methylated CpG sites with each region as well as the genes overlapping these regions (when applicable) are also detailed. All regions with a positive mean differential coefficient are hypermethylated regions while regions with negative mean differential coefficients are hypomethylated. The last two columns list the minimum smoothed false discovery rate (FDR), and the Holm-adjusted FDR (HMFDR) which was used to determine significance.