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. 1968 Feb;106(3):759–766. doi: 10.1042/bj1060759

Changes of total water and sucrose space accompanying induced ion uptake or phosphate swelling of rat liver mitochondria

E J Harris 1, K Van Dam 1
PMCID: PMC1198569  PMID: 5639931

Abstract

1. Total water exchangeable with tritiated water and sucrose space were measured in rat liver mitochondria during the uptake of K+ induced by valinomycin and the release caused by nigericin. The K+ content and the sucrose-inaccessible water rose and fell together. 2. Swelling resulting from phosphate addition in a medium of high K+ concentration was associated mainly with increased sucrose-accessible water, which carried dissolved K+. This change was reversed by addition of ATP. 3. The response of the sucrose-inaccessible space to changed osmolarity was qualitatively that expected if the mitochondrial K+ is assumed to be present in this space with a univalent anion. 4. It is brought out that the light-scattering method fails to distinguish between changes in sucrose space and in sucrose-inaccessible space, which in the present experiments could be altered respectively by phosphate (in high K+ solution) and by cation uptake induced by antibiotic.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AMOORE J. E., BARTLEY W. The permeability of isolated rat-liver mitochondria to sucrose, sodium chloride and potassium chloride at 0 degrees. Biochem J. 1958 Jun;69(2):223–236. doi: 10.1042/bj0690223. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BARTLEY W. Solute movemetns during volume changes in rat-liver mitochondria. Biochem J. 1961 Jul;80:46–57. doi: 10.1042/bj0800046. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bartley W., Enser M. B. The swelling and contraction of isolated rat-liver mitochondria. Biochem J. 1964 Nov;93(2):322–330. doi: 10.1042/bj0930322. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cockrell R. S., Harris E. J., Pressman B. C. Energetics of potassium transport in mitochondria induced by valinomycin. Biochemistry. 1966 Jul;5(7):2326–2335. doi: 10.1021/bi00871a022. [DOI] [PubMed] [Google Scholar]
  5. Graven S. N., Estrada-O S., Lardy H. A. Alkali metal cation release and respiratory inhibition induced by nigericin in rat liver mitochondria. Proc Natl Acad Sci U S A. 1966 Aug;56(2):654–658. doi: 10.1073/pnas.56.2.654. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Harris E. J., Catlin G., Pressman B. C. Effect of transport-inducing antibiotics and other agents on potassium flux in mitochondria. Biochemistry. 1967 May;6(5):1360–1369. doi: 10.1021/bi00857a019. [DOI] [PubMed] [Google Scholar]
  7. Harris E. J., Cockrell R., Pressman B. C. Induced and spontaneous movements of potassium ions into mitochondria. Biochem J. 1966 Apr;99(1):200–213. doi: 10.1042/bj0990200. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. LEHNINGER A. L. Phosphorylation coupled to oxidation of dihydrodiphosphopyridine nucleotide. J Biol Chem. 1951 May;190(1):345–359. [PubMed] [Google Scholar]
  9. LEHNINGER A. L. Reversal of thyroxine-induced swelling of rat liver mitochondria by adenosine triphosphate. J Biol Chem. 1959 Aug;234(8):2187–2195. [PubMed] [Google Scholar]
  10. LEHNINGER A. L. Reversal of various types of mitochondrial swelling by adenosine triphosphate. J Biol Chem. 1959 Sep;234:2465–2471. [PubMed] [Google Scholar]
  11. LEHNINGER A. L. Water uptake and extrusion by mitochondria in relation to oxidative phosphorylation. Physiol Rev. 1962 Jul;42:467–517. doi: 10.1152/physrev.1962.42.3.467. [DOI] [PubMed] [Google Scholar]
  12. MALAMED S., RECKNAGEL R. O. The osmotic behavior of the sucrose-inaccessible space of mitochondrial pellets from rat liver. J Biol Chem. 1959 Nov;234:3027–3030. [PubMed] [Google Scholar]
  13. RACKER E. Mechanisms of synthesis of adenosine triphosphate. Adv Enzymol Relat Subj Biochem. 1961;23:323–399. doi: 10.1002/9780470122686.ch7. [DOI] [PubMed] [Google Scholar]
  14. WERKHEISER W. C., BARTLEY W. The study of steady-state concentrations of internal solutes of mitochondria by rapid centrifugal transfer to a fixation medium. Biochem J. 1957 May;66(1):79–91. doi: 10.1042/bj0660079. [DOI] [PMC free article] [PubMed] [Google Scholar]

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