Fig. 2.

Effect of linalool on E. miricola morphology. (A) The effects of linalool on the viability of E. miricola were observed using PI staining assay. Control (without linalool), 0.5 × MIC (0.0625 %) and 1 × MIC (0.125 %) of linalool treatment. (B) SEM images of E. miricola treated and untreated with linalool. Untreated bacteria (Control group); 1 × MIC of linalool treated bacteria for 20, 40 and 60 min (Experimental group). The image on the right was an enlarged view of the red rectangle on the left image, with red arrows indicating the folded surfaces of the cells. (C) florescent microscopy images of E. miricola treated with linalool for 0, 30, 60, 90, and 120min. White arrows indicated fluorescence penetrating into the cells.