TABLE 1.

Sequences of PCR primers and sequence-specific probes for macaque cytokines

Cytokine	Primer or probe	Sequence	Corresponding cDNA sequence	Product length (bp)
IL-1βa	Forward primer	5′TCACTGCACTGCACACTCCG3′	361-380	101
	Reverse primer	5′TGCTCCAGATCCTGTCCCTG3′	442-461
	Probe	5′-FAM-AAGCTTGGTGATGTCTGGTCCATATGAACTGAAAG-TAMRA-3′	396-430
IL-8a	Forward primer	5′AGAGTGGACCACACTGTGCC3′	217-236	138
	Reverse primer	5′ATGGATTTTGATTCTCAGCCC3′	334-354
	Probe	5′-FAM-TGGGTGCAGAGGGTTGTGGAGAA-TAMRA-3′	300-322
IL-6a	Forward primer	5′CACACAGCCAGCCACTGACC3′	125-144	137
	Reverse primer	5′TTCTGCCAGTGCCTCTTTGC3′	242-261
	Probe	5′-FAM-CACATTCGGTACATCCTCGACGGCATCT-TAMRA-3′	166-191
TNF-αa	Forward primer	5′CAGCCTCTTCTCCTTCCTGC3′	127-146	143
	Reverse primer	5′AAGATGATCTGACTGCCTGAGC3′	248-269
	Probe	5′-FAM-CCACGCTCTTCTGTCTGCTGCACTTT-TAMRA-3′	162-187
MIP-1αb	Forward primer	5′ACTACTTGGAGACGAGCAGCC3′	86-106	114
	Reverse primer	5′CGCTGACATATTTCTGGACCC3′	179-199
	Probe	5′-FAM-CTCCAAGCCCGGTGTCATCTTCCTAA-TAMRA-3′	111-136
IL-10a	Forward primer	5′CTTGCTGGAGGACTTTAAGGG3′	214-234	116
	Reverse primer	5′GCTCCTTGATGTCTGGGTCG3′	310-329
IFN-βa	Forward primer	5′CAACATGACCAACAAGTGTCTCC3′	24-46	122
	Reverse primer	5′TTGTAGGAATCCAAGCAAGTTGTAGC3′	120-145

^aBased on sequences deposited in GenBank. Accession numbers: IL-1β, U19845; IL-8, U19849; IL-6, AB000554; TNF-α, AB000513; IL-10, L26029; IFN-β, AJ011909.

^bBased on an unpublished sequence obtained from Francois Villinger.