FIG. 3.

Immunoblotting using whole bacterial lysates and the serum from an IBD patient. The whole bacterial lysate obtained from TS2, B. ovatus JCM 5824, B. vulgatus JCM 5826, or E. coli JCM 1649 was loaded in each lane as shown at the top. In the two lanes on the far left, the lysates were treated by 2-ME before being loaded onto the gel. The numbers indicated in the left margin of the gel represent the molecular masses (in thousands) as determined by the mobilties of the molecular mass markers. The serum from a CD patient (patient 2) was used as an antibody for the reaction.