Figure 5. Exclusion of Trypan Blue in cells.

Cells were ATP-depleted for 3 h and then incubated with 0.2% TB in PBS at 4 °C for 15 min. The cytosol stained by TB represents the loss of plasma-membrane integrity and cell death. The positive-staining cells were counted under an optical microscope. Results are means±S.D. (n=6; **P<0.01 versus ATP-depleted cells in the absence of glycine).