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. 2005 Aug 23;390(Pt 2):475–484. doi: 10.1042/BJ20050233

Figure 6. Gel-filtration analysis of complexes between chLBD and hLep (humLEP) or oLep (chLep) on a Superdex™ 75 HR 10/30 column.

Figure 6

Complex formation was achieved by 20 min incubation at room temperature in TN buffer using various molar ratios; 200 μl aliquots of the mixture were applied to the column, preequilibrated with the same buffer. The initial hormone concentration (10 μM) was constant in all cases. The column was developed at 0.8 ml/min and calibrated with BSA [66 kDa, RT=11.47 min], ovine placental lactogen (23 kDa, RT=14.5 min) and lysozyme (14 kDa, RT=21.45 min). Protein concentration in the eluate was monitored by measuring the absorbance at 220 nm.