Figure 9. inding determinants of chLBD with oLep.

(A) Tube presentation of the chLBD indicating the segments that, upon mutations, abolished binding towards oLep completely. These segments include Phe⁷³ located in L3 (red), residues 76–79 from L3 (blue) and residues 105–106 located in L4 that links the two domains (magenta). (B) Surface presentation of chLBD where the molecule is rotated by 90° clockwise along the vertical axis with reference to the orientation on the left-hand side. The colour code indicates the effect of the mutations on oLep binding. Red, magenta and blue colours designate no binding, substantial and moderate reductions in binding affinities respectively according to the results displayed in Table 4.