Table 3. Kinetic constants measured by SPR for complex formation of hLBD and LBD with hLep.
Immobilized ligand | Injected ligand | kon (mol−1·s−1×105) | koff (s−1×10−3) | Kd (M×10−8) |
---|---|---|---|---|
hLep | hLBD | 1.20±0.23 | 1.85±0.30 | 1.54 |
chLBD | 1.83±0.95 | 4.25±3.40 | 2.32 | |
chLBD | hLep | 1.68±0.30 | 0.74±0.23 | 0.44 |