Figure 1. Structural analysis of the GLCE promoter.

(A) Identification of the transcriptional start site of the human GLCE gene. The arrow points to the largest transcript generated by an RT from a primer anchored in the 5′-UTR of the gene. (B) Mapping of the cis-acting transcriptional factor binding sites on the human GLCE gene promoter. Only transcriptional factor-binding sites that are conserved at the same position in the human and mouse promoter sequences are indicated. The acronyms used to identify the ligands are: c-myb, protoncogene, required for G₁/S transition; Lef1–TCF4, β-catenin–TCF4 complex; NFAT, nuclear factor of activated T-cells [activated by CAML (calcium-modulator cyclophilin ligand)]. A part of the sequence (+52/+70), complementary to the oligonucleotide used as the primer to identify the transcriptional start sites, is also highlighted.