Figure 2. Efficiency of the Ni²⁺ column in the purification of NDH-1 complexes.

(A) Complexes were isolated from the NdhL-His strain using the Ni²⁺ affinity column, separated by SDS/PAGE, electroblotted to a PVDF membrane and probed with anti-NdhJ antibody. In (B) the CupA-His strain was used and the membrane was probed with anti-NdhF3 antibody. Thylakoid proteins equivalent to 50 mg were solubilized and applied to the Ni²⁺ affinity column. Equal relative volumes were loaded on the wells. Lane 1, initial sample; lane 2, unbound material; lane 3, eluted material.