Table 1. Subunits of NDH-1 from T. elongatus identified by MALDI-TOF.

The spot number corresponds to that in Figure 3. ORF, the open reading frame in CyanoBase. M, molecular mass. Experimental molecular-masses were estimated by comparison of the migration of the respective proteins with that of molecular-mass markers. MALDI-TOF MS results are presented as the ratios of peptide masses providing the identity with the total amount of peptides used in the search, percentages of coverage for full-length proteins and score values according to Mascot.

			M (kDa)
Spot no.	NDH-1 subunit	ORF	Theoretical	Experimental	Peptides matched/total	Cover (%)	Score
2	CupA	tlr0906	51.0	49	18/24	43	277
4	NdhH	tlr1288	45.5	41	20/26	57	209
9	NdhK	tlr0705	25.9	24.5	6/9	26	85
10	NdhI	tlr0668	22.9	24	12/16	55	138
12	Tll0220	tll0220	15.8	20.2	4/7	47	76
13	NdhJ	tlr1430	19.4	20	12/17	83	187
14	NdhN	tlr1130	16.6	15.5	7/9	57	110
15	NdhM	tll0447	12.6	13	5/6	45	73