Skip to main content
. 2005 Aug 23;390(Pt 2):521–528. doi: 10.1042/BJ20050439

Figure 4. A role for NFE2 sites in basal human GSS promoter activity.

Figure 4

(A) EMSA demonstrating binding of Chang nuclear proteins (5, 10 and 15 μg) to both the proximal and distal NFE2 sites. Specificity of binding is determined by competition of binding with 100-fold excess unlabelled probe. (B) Mutagenesis of proximal and distal NFE2 sites within the full-length promoter decreases basal promoter activity by 66%. Relative luciferase activity was measured following 24 h transfection with wild-type full-length GSS and full-length GSS promoter with NFE2 site mutations. Values are expressed as fold-wild-type luciferase activity and represent three experiments performed in triplicate. *P<0.05 versus wild-type construct.