Figure 2. DSC of SOPC:cholesterol (6:4) or SOPC:ent-cholesterol (6:4) with 0.2 mol% PtdIns(4,5)P₂ with or without 10 mol% NAP-22 peptide.

The scan rate was 2K/min, and the lipid concentration was 2.5 mg/ml in 20 mM Pipes containing 1 mM EDTA and 150 mM NaCl supplemented with 0.002% NaN₃, pH 7.4. Sequential heating and cooling scans was performed between 0 and 50 °C. Numbers are the order in which the scans were carried out, with scans 1, 3 and 5 being heating scans, each of which was followed by one of the cooling scans 2, 4 or 6. Scans are displaced along the y-axis for clarity of presentation. myr, myristoylated; PI, PtdIns; 1 cal=4.184 J.