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| Enzyme | Substrate | kcat (s−1) | Km (μM) | kcat/Km (mM−1·s−1) |
|---|---|---|---|---|
| Wild-type | R,R-TSO | 23±2 | 10±1 | 2400±210 |
| Y154F | R,R-TSO | 0.08±0.008 | 21±4.8 | 4±0.5 |
| Y235F | R,R-TSO | 0.07±0.01 | 50±13 | 1.3±0.2 |
| H300A | R,R-TSO | 0.04±0.008 | 21±7 | 2±0.3 |
| H300Q | R,R-TSO | 0.02±0.007 | 66±34 | 0.3±0.05 |
| Wild-type | S,S-TSO | 2.6±0.1 | 0.7±0.2 | 3700±870 |
| Wild-type | rac-TSO | 3.9±0.2 | 1.6±0.3 | 2400±380 |
| Wild-type | 4-NSO | 3.1±0.5 | 510±110 | 6±2 |
