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| Enzyme | Substrate | KS (μM) | k2 (s−1) | k−2 (μM·s−1) | k2/KS (s−1) | k3 (s−1) |
|---|---|---|---|---|---|---|
| Wild-type | R,R-TSO | >30 | >100 | 47±6 | 2.7±0.3 | 24±3 |
| H300N | R,R-TSO | 60±40 | 0.3±0.06 | 0.13±0.02 | 0.005±0.002 | 0.003±0.0005 |
| Wild-type | S,S-TSO | 11±6 | 18±2 | 14±2 | 1.8±1.4 | 3±0.1 |
| H300N | S,S-TSO | >40 | >0.035 | 0.05±0.004 | 0.006±0.0002 | − |
