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. 2002 Jan;9(1):176–181. doi: 10.1128/CDLI.9.1.176-181.2002

FIG. 3.

FIG. 3.

Western blot analyses of G5 i-antigen. Lanes 1, reduced purified G5 i-antigen (0.7 μg); lanes 2, reduced G5 membrane protein sample (5 μg); lanes 3, nonreduced G5 membrane protein sample (5 μg); lanes 4, nonreduced purified G5 i-antigen (0.7 μg). (A) The probe was rabbit anti-G5 i-antigen serum (1:5,000); (B) the probe was MAb G3-61 (1:1,000); (C) the probe was fish anti-G5 i-antigen serum (1:500). The secondary antibodies are goat anti-rabbit IgG, rabbit anti-mouse IgG, and mouse anti-catfish Ig MAb (MAb MAF13) conjugated with alkaline phosphatase, respectively. Rabbit antiserum against purified G5 i-antigen recognizes both reduced and nonreduced G5 i-antigens. Immobilizing MAb G3-61 and fish anti-G5 i-antigen serum recognize only the nonreduced forms of the protein.