FIG. 4.

Real-time PCR amplification plot of the four isoforms of PPT-A cDNA. A reading of a change in fluorescence (Rn) as a function of the cycle numbers is demonstrated for a range of known input copy numbers of plasmids containing the four isoforms of PPT-A. Tenfold serial dilutions of the four plasmids were amplified. The input amounts of targets were 30 to 3 × 10⁶ copies per reaction mixture for plasmids containing the α, β, γ, and δ isoforms of PPT-A, as indicated. NC, negative control, which lacked PCR-amplified product when the template was omitted.