TABLE 4.

Intra-assay reproducibility of SP real-time PCR assay^a

Repetition	CT
	PBLs	MDMs	Microglia	NT-2N cells
1	39.71	39.78	30.86	26.0
2	39.82	39.67	30.86	25.65
3	39.75	39.55	30.75	25.67
4	39.80	39.51	30.81	25.58
Mean ± SD	39.77 ± 0.05	39.63 ± 0.12	30.82 ± 0.05	25.73 ± 0.19
CV (%)	0.12	0.31	0.17	0.73

^aFour cDNA samples from each cell type (PBLs, MDMs, microglia, and NT-2N cells) were amplified by the SP real-time PCR assay.