Table 5.

Real-time polymerase chain reaction (PCR) protocol.

Polymerase activation and DNA denaturation		98°C / 1 min.
Amplification	Denaturation	95°C / 15 sec.	40 cycle
	Annealing/Extension	60°C / 60 sec.
Melting curve analysis	Increase 1°C for each step between 60 °C-95 °C
	Hold for 90 seconds on first step / Hold for 5 seconds on other steps