Table 1.

Central composite design affecting pectinase activity of G. candidum AHC1.

Run order	Factors
	Temperature (°C)	pH	Substrate concentration (%)	Reaction time (min)	Pectinase activity (IU mL −1 ) a
1	29.8	4.6	1.3	18	40
2	32.2	4.6	1.3	18	70.1
3	29.8	5.4	1.3	18	44.5
4	32.2	5.4	1.3	18	68.3
5	29.8	4.6	1.7	18	57.4
6	32.2	4.6	1.7	18	56.9
7	29.8	5.4	1.7	18	60
8	32.2	5.4	1.7	18	77.1
9	29.8	4.6	1.3	22	47.4
10	32.2	4.6	1.3	22	67.9
11	29.8	5.4	1.3	22	6.21
12	32.2	5.4	1.3	22	67
13	29.8	4.6	1.7	22	30.8
14	32.2	4.6	1.7	22	57.4
15	29.8	5.4	1.7	22	35.1
16	32.2	5.4	1.7	22	66.4
17	31	5	1.5	20	1.7
18	31	5	1.5	20	1.6
19	31	5	1.5	20	1.2
20	25	5	1.5	20	6.2
21	37	5	1.5	20	15.33
22	31	3	1.5	20	27.6
23	31	7	1.5	20	32
24	31	5	0.5	20	1.7
25	31	5	2.5	20	11
26	31	5	1.5	10	31.9
27	31	5	1.5	30	2.4

^aInsignificant standard deviation.