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. 2025 Mar 7;2025:8853746. doi: 10.1155/ijfo/8853746

Table 2.

Analysis of variance of central composite design for pectinase activity by G. candidum AHC1.

Source DF Adj. SS Adj. MS F value p value
Model 15 15452.4 1030.16 4.88 0.006
Blocks 1 8129.7 8129.75 38.53 < 0.001
Linear 4 1980.4 495.09 2.35 0.119
Temperature 1 988.1 988.12 4.68 0.053
pH 1 5.3 5.31 0.03 0.877
Substrate concentration 1 87.9 87.94 0.42 0.532
Reaction time 1 899.0 898.99 4.26 0.063
Square 4 7398.6 1849.64 8.77 0.002
Temperature∗temperature 1 6400.9 6400.91 30.34 < 0.001
pH∗pH 1 7189.0 7188.97 34.07 < 0.001
Substrate concentration∗substrate concentration 1 6224.5 6224.46 29.50 < 0.001
Reaction time∗reaction time 1 6660.0 6660.05 31.57 < 0.001
Two-way interaction 6 1309.9 218.32 1.03 0.453
Temperature∗pH 1 198.0 197.99 0.94 0.353
Temperature∗substrate concentration 1 230.2 230.16 1.09 0.319
Temperature∗reaction time 1 294.8 294.84 1.40 0.262
pH∗substrate concentration 1 356.1 356.11 1.69 0.220
pH∗reaction time 1 184.2 184.17 0.87 0.370
Substrate concentration∗reaction time 1 46.6 46.64 0.22 0.647
Error 11 2320.8 210.98
Lack-of-fit 9 2320.7 257.85 3683.60 < 0.001
Pure error 2 0.1 0.07
Total 26

Note: Model summary: S = 14.5252; R‐sq. = 86.94%; R‐sq.(Adj.) = 69.14%; R‐sq.(Pred.) = 0.00%.