Table 2.
Analysis of variance of central composite design for pectinase activity by G. candidum AHC1.
| Source | DF | Adj. SS | Adj. MS | F value | p value |
|---|---|---|---|---|---|
| Model | 15 | 15452.4 | 1030.16 | 4.88 | 0.006 |
| Blocks | 1 | 8129.7 | 8129.75 | 38.53 | < 0.001 |
| Linear | 4 | 1980.4 | 495.09 | 2.35 | 0.119 |
| Temperature | 1 | 988.1 | 988.12 | 4.68 | 0.053 |
| pH | 1 | 5.3 | 5.31 | 0.03 | 0.877 |
| Substrate concentration | 1 | 87.9 | 87.94 | 0.42 | 0.532 |
| Reaction time | 1 | 899.0 | 898.99 | 4.26 | 0.063 |
| Square | 4 | 7398.6 | 1849.64 | 8.77 | 0.002 |
| Temperature∗temperature | 1 | 6400.9 | 6400.91 | 30.34 | < 0.001 |
| pH∗pH | 1 | 7189.0 | 7188.97 | 34.07 | < 0.001 |
| Substrate concentration∗substrate concentration | 1 | 6224.5 | 6224.46 | 29.50 | < 0.001 |
| Reaction time∗reaction time | 1 | 6660.0 | 6660.05 | 31.57 | < 0.001 |
| Two-way interaction | 6 | 1309.9 | 218.32 | 1.03 | 0.453 |
| Temperature∗pH | 1 | 198.0 | 197.99 | 0.94 | 0.353 |
| Temperature∗substrate concentration | 1 | 230.2 | 230.16 | 1.09 | 0.319 |
| Temperature∗reaction time | 1 | 294.8 | 294.84 | 1.40 | 0.262 |
| pH∗substrate concentration | 1 | 356.1 | 356.11 | 1.69 | 0.220 |
| pH∗reaction time | 1 | 184.2 | 184.17 | 0.87 | 0.370 |
| Substrate concentration∗reaction time | 1 | 46.6 | 46.64 | 0.22 | 0.647 |
| Error | 11 | 2320.8 | 210.98 | ||
| Lack-of-fit | 9 | 2320.7 | 257.85 | 3683.60 | < 0.001 |
| Pure error | 2 | 0.1 | 0.07 | ||
| Total | 26 |
Note: Model summary: S = 14.5252; R‐sq. = 86.94%; R‐sq.(Adj.) = 69.14%; R‐sq.(Pred.) = 0.00%.