Table 2.

Comparison of SHIFT-SP protocol with MagMAX™ DNA Multi-Sample Kit and QIAamp® DNA Blood Mini Kit.

NA extraction method	Average total yield in ng	Method TAT&	Eluate volume	Concentration of NA in the final eluate
Experiment 1: SHIFT-SP (n = 4)	112.8 (24.5)	~ 6 – 7 min	20 µL*	5.64 ng/µL
Experiment 2: MagMAX™ DNA Multi-Sample Kit (n = 3)	105.7 (5.0)	~ 36 min	200 µL	0.53 ng/µL
Experiment 3: QIAamp® DNA Blood Mini Kit (n = 3)	57.8 (6.2)	~ 14 – 15 min	200 µL	0.29 ng/µL

The yield from SHIFT-SP is comparable with MagMAX™ DNA multi-sample kit protocol, where nearly all the spiked DNA (100 ng) is retrieved after extraction. SHIFT-SP is the shortest of the three and yields a concentrated sample. Error values in brackets are SD. *10 µL eluate is mixed with 10 µL elution wash. ^&Time from lysed sample to NA. p-value 0.31 is not significant between average total yields of SHIFT-SP and MagMAX™ DNA Multi-Sample kit. p-value 0.01 is significant between average total yields of SHIFT-SP and QIAamp® DNA Blood mini Kit.