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. 1983 Jul;340:503–511. doi: 10.1113/jphysiol.1983.sp014776

Aldosterone on sodium transport of rat distal colon in long-term adrenalectomy during acute and chronic substitution

Michael Horster 1, Andreas Lückhoff 1
PMCID: PMC1199223  PMID: 6887059

Abstract

1. The influence of aldosterone upon water and sodium transport properties of the distal colon was studied in long-term adrenalectomy (11-29 days).

2. Six groups of rats were used: I, normal (control); II, adrenalectomized; III, adrenalectomized, acutely substituted with aldosterone (200 μg/kg 4 h); IV, adrenalectomized rats receiving aldosterone simultaneously with the specific inhibitor spironolactone (40 mg/kg within 4 h); V, adrenalectomized, substituted chronically with aldosterone (2 × 75 μg/kg day); VI, adrenalectomized, substituted chronically with dexamethasone (120 μg/kg day).

3. Distal colon segments were perfused in vivo with isotonic Ringer solution. In addition, a hypotonic electrolyte solution (Na+ 111 mM) was used in groups I and II.

4. In adrenalectomy (group II), net water absorption (Jv) was significantly decreased from (normal) 54·4 μl/h cm2±10·5 (n = 9) to 41·2 μl/h cm2±7·3 (n = 4), and net Na+ absorption (JNa) was decreased from 13·6 μmol/h cm2+3·5 to 8·5 μmol/h cm2±0·9 (isotonic perfusate). Similarly, Jv was decreased from 54·0 μl/h cm2±8·3 (n = 4) to 37·3 μl/h cm2±4·2 (n = 7), and JNa from 8·6 μmol/h cm2±2·1 to 4·2 μmol/h cm2±2·1 (hypotonic perfusate).

5. Acute aldosterone substitution in adrenalectomy (III) had no effect upon Jv (37·1 μl/h cm2±10·3; n = 5) but increased JNa to 10·3 μmol/h cm2±0·3.

6. The luminal Na+ steady-state concentration was higher in group II (11·2 mmol l-1±3·6; n = 6) than in group I (3·3 mmol l-1±1·4; n = 29). Acute aldosterone substitution restored this value to normal (3·0 mmol l-1±1·2; n = 4). The aldosterone effect was partly blocked by spironolactone: the Na+ steady-state concentration was 6·4 mmol/l±0·6 (n = 3) in group IV.

7. At the steady-state luminal Na+ concentration, the osmotically driven net water fluxes were not different in groups I and II, indicating that the hydraulic permeability coefficient is not altered in adrenalectomy.

8. In group V, Jv (54·9 μl/h cm2±10·9; n = 7) and JNa (11·9 μmol/h cm2±1·7; n = 6) were not significantly different from normal.

9. In group VI, Jv (37·3 μl/h cm2±6·0; n = 5) and JNa (8·0 μmol/h cm2±1·4) were not significantly different from group II.

10. The mineralocorticoid effects of aldosterone in long-term adrenalectomy appear to represent the principal determining factors of colonic Jv and JNa.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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