FIG. 1.

CD4⁺ and CD8⁺ T cells must be segregated for TCR CDR3 analysis. CD4⁺ sorted T cells (top), CD8⁺ sorted T cells (middle), or unseparated T cells (bottom) were subjected to TCR CDR3 analysis. As a paradigm, the TCR VA23 and the VCR VB7 analyses from TIL are depicted. Note that the rather monoclonal TCR VA23 peak in the CD4⁺ T-cell pool would be missed if nonsorted T cells had served as the source to generate cDNA for TCR CDR3 analysis.