TABLE 2.

Monoclonal TCR transcripts^a

Patient	Source	V family	V region	CDR3 region	Joining region	J family
MZ-CC01-1	CD8+ PBL	VA 9	SAMYYCA	PASRS	NTGKLIFGQG	AJ 37
		VA 19	SAVYICA	VDSR	AAGNKLTFGGG	AJ 17
		VA 22	SAVYFCA	LSPI	SSGSARQLTFGSG	AJ 22
	CD4+ TIL	VA 2	SATYLCA	VNTY	SGNTPLVFGKG	AJ 29
		VA 5	SATYLCA	LDQG	DYKLSFGAG	AJ 20
		VA 6	SAMYFCA	MREGY	SGNTPLVFGKG	AJ 29
		VA 7S2	SASYLCA	VREQ	AAGNKLTFGGG	AJ 17
		VA 12	SAVYFCA	PL	TNAGKSTFGDG	AJ 27
		VA 13	SGVYFCA	V	SGYSTLTFGKG	AJ 11
		VA 21	SAVYFCA	AKD	QAGTALIFGKG	AJ 15
		VA 24	SASYICV	VP	NTDKLIFGTG	AJ 34
		VB 5S8	SALYLCA	SSFRIGY	GYTFGSG	BJ 1-2
		VB 17	TAFYLCA	SREWGD	TEAFFGQG	BJ 1.1
MZ-CC01-2	CD8+ PBL	VA 5	SATYLCA	LDSR	AAGNKLTFGGG	AJ 17
		VA 8S2	SAVYFCA	ENTF	NQGGKLIFGQG	AJ 23
		VA 17	SATYFCA	ATYV	NSGGGADGLTFGKG	AJ 45
	CD4+ TIL	VA 2	SATYLCV	VPDGI	NDMRFGAG	AJ 43
		VA 13	SGVYFCA	VERW	ARLMFGDG	AJ 31
		VA 20	TAVYYCL	VGPLM	NSGGYQKVTFGIG	AJ 13
		VA 22	SAVYFCA	LKGR	NNNDMRFGAG	AJ 43
		VA 23	SATYLCA	VR	SGAGSYQLTFGKG	AJ 28
	Tumor	VA 16	SALYFCA	VRDHS	SGGYNKLIFGAG	AJ 4
		VB 7S1	SALYLCA	SSQEGDL	YEQYFGPG	BJ 2-7
		VB 15	TALYFCA	TSDSDSGD	TDTQYFGPG	BJ 2-3
		VB 23	SALYFCA	SSQDN	SYEQYFGPG	BJ 2-7
MZ-CC01-3	CD8+ PBL	VA 13	SGVYFCA	VDAGY	GNEKLTFGTG	AJ 48

^acDNA was generated from CD4⁺ and CD8⁺ PBL, freshly harvested tumor tissue, and CD4⁺ TIL and tested for monoclonal TCR VA or VB transcripts as described in the text. TCR VA or VB families were characterized for monoclonality by direct sequencing of the amplified product from each TCR family. Note the large number of monoclonal TCR VA mRNA transcripts in CD4⁺ TIL from patient MZ-CC01-1. Monoclonal TCR transcripts detected in freshly harvested tumor tissue could not be detected in TIL or in PBL. Thus, individual TCR clonotypes could not be expanded in vitro. Short-term (7 days) in vitro culture of T cells (TIL) in the presence of autologous tumor and IL-7 resulted in an oligoclonal T-cell population.