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. 2024 Oct 11;20(2):811–818. doi: 10.1016/j.jds.2024.09.021

Figure 3.

Figure 3

Pretreatment of Poly (I:C) reduced apoptosis induced by P. gingivalis in HUVECs. (A, B) Following pretreatment with Poly (I:C) or its absence, P. gingivalis stimulation was assessed, and protein expression levels of Caspase 3, Caspase 9, Bcl-2 and Bax were evaluated using Western blot analysis. (C) Following pretreatment with Poly (I:C) or without, P. gingivalis was stimulated, and HUVECs apoptosis was assessed using flow cytometry. (D) Statistical analysis of Flow Cytometry. Dual staining with FITC and PI identifies apoptotic cells, whereas single staining with FITC specifically marks early apoptotic cells. The cumulative count of both reflects the total population of apoptotic cells. ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001. Poly (I:C), Polyinosinic-polycytidylic acid; P. gingivalis, P. gingivalis; PBS, Phosphate buffered saline; Bcl-2, B-Cell Lymphoma-2; Bax, Bcl2-associated x; FITC, Fluorescein isothiocyanate; PI, Propidium iodide.