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LOD, sensitivity, and specificity of the studied approaches.
| Techniqes | LOD | Sensitivity | Specificity |
|---|---|---|---|
| Real-time PCR (reference) | 15 copies/μL | 100% | 100% |
| RT–LAMP | 15 copies/μL | 100% | 98% (two false positives out of 100) |
| Nested–RT–LAMP | 5 copies/μL | 100% | 98% (two false positives out of 100) |
| Extraction-free nested–RT–LAMP | — | 94% (six false negative out of 100) | 97% (three false positives out of 100) |
