Table 1.
Sequences of the Primers Used in the Present Study
|
Amplification Primers |
Sequencing Primers |
|||
| Use for Primers | Forward | Reverse | Forward | Reverse |
| rs6100157 | TTGGCAGATAACTGCTGTGG | AGGCCCGGGCAGCACTCAC | ACTGCTGTGGACGGATTAA | AATCCAAAATGCTCCAATG |
| rs2296524 | CCTGAAGTCCTCCAGCTGTC | GGGGTGGAGGGAGAGTAAAG | GCTTCTCTCCCAGGCTTGC | AGGCCCGGGCAGCACTCAC |
| rs6100158 | GGGCACCAAGATAGTTCAGC | GGGGTGGAGGGAGAGTAAAG | GTTTCACCCAGCAGACCTT | … |
| Isoform B of STX16 | (G): AGCCCTCTGCATTCACGTAG | (H): TATCGAGACTTCACGCCAAC | … | CCGATGGTAAAGAGTGTTATCGa |
| PCR across the 4.4-kb microdeletion | (A): TGAGGGGAACACATGCATAA | (B): CCGATGGTAAAGAGTGTTATCG | GGAGCAGGAGTGTTGAGGAA | … |
| Multiplex PCR for detection of the 4.4-kb microdeletion | (C): GGAGCAGGAGTGTTGAGGAA; (E): TGTCCACGGATGGTCAGTAA | (D): CAGGGCCAGTGTCATTTCTT; (F): AAAGGTGCCAATCACTCCAC | … | … |
a
This primer was used to sequence the polymorphic nucleotide 527 of STX16 isoform B, which corresponds to rs2296524.