FIG. 4.
GSE does not modulate IL-6-specific gene expression by normal PBMC. PBMC (3 × 106 cells/ml) were cultured alone or with different concentrations of GSE (0.01 [lanes 2], 0.1 [lanes 3], and 0.5 [lanes 4] mg/ml) for 48 h. Cytoplasmic RNA was extracted, reverse transcribed, and amplified with specific primers for housekeeping gene β-actin (A) and IL-6 and IFN-γ (B). Additional lanes: MW, molecular weight markers; 1, control. Also shown are quantitation of changes in IL-6 (C) and IFN-γ (D) gene expression. Data are means ± SD (error bars) of four experiments performed in triplicate using PBMC from four different subjects. Results showed that GSE upregulated IFN-γ gene expression, while IL-6 gene expression was not modulated in the same RNA samples. The statistical significance of the differences between control and treated samples was calculated by Student's t test.