FIG. 5.

GSE does not induce IL-6 synthesis and secretion by normal PBMC. Supernatants from GSE-treated and control cultures (24 to 72 h) were assayed for IL-6 by a quantitative sandwich ELISA technique using the Quantikine kit (R & D Systems) as described by the manufacturer. Data are means ± SD (error bars) of four experiments performed in triplicate using PBMC from four different healthy subjects. Statistical significance of the differences between control and GSE-treated cultures was evaluated by Student's t test. These same culture supernatants were also quantitated for IFN-γ levels, and GSE at 0.1 and 0.5 mg/ml produced significant levels of IFN-γ compared to untreated control cultures, similar to results reported in Fig. 3 (these data are not included in Fig. 5 for clarity).