Figure 1.
Determination of TSSs utilized by the PD1 class I gene in vitro and in vivo. (A) In vitro transcription of the PD1 class I promoter/reporter construct, -416WT. (Lane 1) HeLa extract alone control; nonspecific background bands are indicated by black filled circles; (lane 2) -416WT template alone; (lane 3) -416WT template in the presence of HeLa extract. Specific TSS are indicated by arrows. A sequence ladder of the class I promoter is provided. (B) Analysis of in vivo TSS by 5′RACE analysis of isolated mRNA from spleen of PD1 class I transgenic mice. (C) TSS generated by the endogenous H-2Kb class I gene in spleens as determined by 5′RACE, using a H-2Kb gene-specific primer with the same mRNA samples as in B. The major TSS regions are indicated by arrows.