Table 4.
Forward primer (Pf)—Ai-Bj-C-D-reverse primer (Pr) | |
---|---|
PfA1B1CDPr | ATAATACCCTCCCACCCA-ATTTCCACCATT-ACCACCCTATAT-ATTCCTCACAAA-AACCATAAACCA-CACCCACCTCCCATAATA |
PfA1B2CDPr | ATAATACCCTCCCACCCA-ATTTCCACCATT-CACACCTTATCT-ATTCCTCACAAA-AACCATAAACCA-CACCCACCTCCCATAATA |
PfA2B1CDPr | ATAATACCCTCCCACCCA-AACACAACTCTT-ACCACCCTATAT-ATTCCTCACAAA-AACCATAAACCA-CACCCACCTCCCATAATA |
PfA2B2CDPr | ATAATACCCTCCCACCCA-AACACAACTCTT-CACACCTTATCT-ATTCCTCACAAA-AACCATAAACCA-CACCCACCTCCCATAATA |
Each target for the DNA computation is listed starting from the 5′ end. Forward (Pf) and reverse (Pr) primers, 18 bases long, bracket the four 12 nt word sequences, ABCD. The two sequences for A (A1 = ATTTCCACCATT and A2 = AACACAACTCTT) are in boldface. The two sequences for B (B1 = ACCACCCTATAT and B2 = CACACCTTATCT) are in italics. The third and forth words, C and D are the underlined sequence, serve as place holders in this computation. The sequences of words used in the computation were generated by a second equivalent DNA library selection.