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Influence of cell depletion on IFN-γ synthesis after H1N1 stimulation in three different experiments on the same donor. Spot numbers correspond to values obtained with PBMC before depletion or after CD4, CD8, or CD56 depletion. Stimulation was done in ELISPOT plates during 24 h by using A/Texas-H1N1 vaccine at a final concentration of 10 μg/ml; 105 cells were used per well, and experiments were performed in triplicate for each condition.
