FIG. 3.
Influence of cell double depletion on IFN-γ synthesis after H1N1 stimulation. Depletion was performed before stimulation with flu vaccine. Spot numbers (for each donor) correspond to values obtained with PBMC before depletion or after CD4-CD8, CD4-CD56, or CD8-CD56 depletion. Stimulation was done in ELISPOT plates during 24 h by using A/Texas-H1N1 vaccine at a final concentration of 10 μg/ml; 105 cells were used per well, and experiments were performed in triplicate for each condition. The results are representative of three independent experiments.