FIG. 4.
Influence of cell depletion on IFN-γ synthesis after H3N2 stimulation. Depletion was performed before stimulation with flu vaccine. Spot numbers (for each donor) correspond to values obtained with PBMC before depletion or after CD4, CD8 or CD56 depletion. Stimulation was done in ELISPOT plates during 24 h by using A/Sydney-H3N2 vaccine at a final concentration of 10 μg/ml; 105 cells were used per well, experiments were performed in triplicate for each condition. The results are representative of at least two independent experiments for each donor.