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Potential regulatory mechanisms involved in IFN-γ secretion after stimulation with H1N1 or H3N2 flu vaccine antigens. Both H1N1 and H3N2 antigens captured and processed by APCs stimulated a CD4 T-cell response, but only H1N1 stimulation triggered NK and CD8-negative roles, directly or indirectly, through CD4 cells. This negative regulation could result from an effect on APCs and/or IFN-γ-producing CD4 cells through direct cell lysis or inhibition of activation.
