Table 2.
List of antibodies used for immunohistochemical studies.
| Antibody | Pre-Treatment | Dilution | Incubation | Cat No. | Source | Control Tissue |
| Glutathione S-Transferase pi GST-π (polyclonal) | None | 1:150 | 30 min RT | PU249-UP | BioGenex (Distributor: Menarini Diagnostics, Wokingham, UK) | Breast Ca |
| P-glycoprotein (MDR-1) (Clone JSB-1) | Pressure Cook 2 min pH 6.0 | 1:100 | Overnight 4°C | NCL-JSB1 | Novo Castra Newcastle-upon-Tyne, UK | Kidney |
| Multidrug Resistant-Related Protein (MRP) (Clone MRPm6, specific for MRP-1) | Pressure Cook 2 min pH 7.0 | 1:30 | 30 min RT | MAB4122 | Chemicon International Chandlers Ford, UK | Kidney |
| Thymidylate Synthase TS (Clone TS 106) | Pressure Cook 2 min pH 6.0 | 1:20 | 30 min RT | MS471P | Neo Markers (Distributor: Lab Vision, Newmarket, UK) | Colon Ca |
When sections required microwaving a Matsui MIIOM microwave was used at 800 W power. Pressure cooking was performed with a Tefal Clipso Pressure Cooker using 70 P power.