Table 1.
Designed species-specific and selected universal oligonucleotide primers used in this study along with amplicon size.
| Species | Primer name |
Primer direction |
Primer size (bp) |
Primer sequence |
Species – Specific amplicon size |
| All | 16s Fwd | Forward | 20 | 5'-CGCCTGTTTATCAAAAACAT-3' | |
| All | 16s Rev | Reverse | 20 | 5'- CTCCGGTTTGAACTCAGATC-3 | ~550 bp |
| Antilope. Cervicapra | BB16 | Reverse | 21 | 5'-ACCTAGTTATTCGCTATCAAG -3' | 410 bp |
| Naemorhedus. goral | GL16 | Reverse | 29 | 5'-GGCAACTAGTTCAAAAAACCTAGTTATTT -3' | 430 bp |
| Boselaphus. tragocamelus | NG16 | Reverse | 24 | 5'-AGTTATTCGCTATCTCACTAAACC -3' | 408 bp |
| Cervus. eldi | TH16 | Reverse | 22 | 5'-CCTAGTTATTTGCTAACACACC -3' | 400 bp |
| Cervus. axis | CH16 | Reverse | 25 | 5'-TATCCCAGAAGGACAGAATAATTAC -3' | 240 bp |
| Cervus. unicolor | SM16 | Forward | 24 | 5'-GCTTTAACTACTTAGCCCAAAGAG -3' | 350 bp |
| Axis. porcinus | HD16 | Reverse | 27 | 5'-CTCACAACAACAAAGGAATCGCTACTC -3' | 300 bp |