Table 2.
Results from species-specific heminested PCR assay with target and non target species for designed primers
| Species | Common Name | DNA source | 1st PCR universal primer 16s rRNA gene | 2nd PCR BB 16 | 2nd PCR GL 16 | 2nd PCR NG 16 | 2nd PCR TH 16 | 2nd PCR CH 16 | 2nd PCR SM 16 | 2nd PCR HD 16 |
| Antilope cervicapra a | Blackbuck | Tissue | + | + | - | - | - | - | - | - |
| Antilope cervicapra b | Blackbuck | Tissue | + | + | - | - | - | - | - | - |
| Antilope cervicapra c | Blackbuck | Blood stain | + | + | - | - | - | - | - | - |
| Boselaphus tragocamelus a | Nilgai | Tissue | + | - | - | + | - | - | - | - |
| Boselaphus tragocamelus b | Nilgai | Tissue | + | - | - | + | - | - | - | - |
| Boselaphus tragocamelus c | Nilgai | Tissue | + | - | - | + | - | - | - | - |
| Naemorhedus goral a | Goral | Blood stain | + | - | + | - | - | - | - | - |
| Naemorhedus goral b | Goral | Tissue | + | - | + | - | - | - | - | - |
| Naemorhedus goral c | Goral | Tissue | + | - | + | - | - | - | - | - |
| Axis porcinus a | Hog deer | Tissue | + | - | - | - | - | - | - | + |
| Axis porcinus b | Hog deer | Tissue | + | - | - | - | - | - | - | + |
| Cervus axis a | Chital | Blood stain | + | - | - | - | - | + | - | - |
| Cervus axis b | Chital | Tissue | + | - | - | - | - | + | - | - |
| Cervus axis c | Chital | Tissue | + | - | - | - | - | + | - | - |
| Cervus axis d | Chital | Tissue | + | - | - | - | - | + | - | - |
| Cervus axis e | Chital | Tissue | + | - | - | - | - | + | - | - |
| Cervus eldi a | Thamin | Tissue | + | - | - | - | + | - | - | - |
| Cervus eldi b | Thamin | Tissue | + | - | - | - | + | - | - | - |
| Cervus unicolor a | Sambar | Tissue | + | - | - | - | - | - | + | - |
| Cervus unicolor b | Sambar | Tissue | + | - | - | - | - | - | + | - |
| Cervus duvaucelii | Barasingha | Tissue | + | - | - | - | - | - | - | - |
| Ovis aries | Sheep | Tissue | + | - | - | - | - | - | - | - |
| Bos tarus | Cow | Tissue | + | - | - | - | - | - | - | - |
| Homo sapiens | Human | Blood | + | - | - | - | - | - | - | - |
| Lepidochelys olevacea | Turtle | Tissue | + | - | - | - | - | - | - | - |
| Hemidactylus fluviviridis | Lizard | Tissue | + | - | - | - | - | - | - | - |
+ positive amplification, - negative amplification