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. 2005 Aug 10;5:14. doi: 10.1186/1471-2229-5-14

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Copyright © 2005 Yoshida et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Arabidopsis ETO1 interacts with LE-ACS3 but not with LE-ACS2 or LE-ACS4 in yeast. Interaction between Arabidopsis ETO1 (in pAS2) and ACC synthases (in pACT2) from tomato (LE-ACS2, LE-ACS3, and LE-ACS4) were analyzed in the yeast two-hybrid system. AtACS5 from Arabidopsis was used as a positive control representing a strong interaction partner with ETO1. pACT2 vector was used as a negative control. Three independent original transformants were analyzed for each combination. Means ± SE (n = 3) are indicated.