Table 2. Plasmids used in the present study.
| Plasmid | Description*† | Source or reference | ||
|---|---|---|---|---|
| pBluescript SK(+)/b12-2 | ClC-2 cDNA | [21] | ||
| pKF14 | CEN | URA3 | PMET25-CLC2-GFP-TCYC1 | Present study |
| pKF16 | CEN | URA3 | PMET25-GFP-CLC2-TCYC1 | Present study |
| pKF19 | CEN | URA3 | PMET25-GEF1-GFP-TCYC1 | [17] |
| pKF20 | CEN | URA3 | PMET25-CLC2-TCYC1 | Present study |
| pKF28 | CEN | URA3 | PMET25-optCLC2-GFP-TCYC1 | Present study |
| pKF31 | CEN | HIS3 | PMET25-optCLC2-TCYC1 | Present study |
| pKF37 | INT | HIS3 | PMET25-optCLC2-TCYC1 | Present study |
| pKF56 | 2μ | TRP1 | PMNT1-MNT1-3×myc | Present study |
| pKF67 | CEN | URA3 | PMET25-KHA1ΔC382-GFP-TCYC1 | Present study |
| pKF68 | CEN | URA3 | PMET25-KHA1-GFP-TCYC1 | Present study |
| pKF76 | CEN | URA3 | PMET25-8×Gly-3×myc-TCYC1 | Present study |
| pKF83 | CEN | URA3 | PMET25-KHA1-8×Gly-3×myc-TCYC1 | Present study |
| pKF86 | CEN | HIS3 | PMET25-GFP-CLC2-TCYC1 | Present study |
| pRS303 | INT | HIS3 | MCS | [22] |
| pRS424 | 2μ | TRP1 | MCS | Stratagene |
| pSM3M-414 | CEN | TRP1 | PMNT1-MNT1-3×myc | S. Munro‡ |
| pUG34 | CEN | HIS3 | PMET25-GFP-MCS-TCYC1 | J. H. Hegemann§ |
| pUG35 | CEN | URA3 | PMET25-MCS-GFP-TCYC1 | J. H. Hegemann§ |
| pUG36 | CEN | URA3 | PMET25-GFP-MCS-TCYC1 | J. H. Hegemann§ |
* Abbreviations for description of plasmids: CEN, centromeric; INT, integrative; 2μ, episomal; MCS, multiple cloning site.
† All listed plasmids convey ampicillin resistance on E. coli cells.
‡ MRC Laboratory of Molecular Biology, Cambridge, U.K.
§ Heinrich-Heine-Universität, Düsseldorf, Germany.