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. 2005 Sep 5;390(Pt 3):681–688. doi: 10.1042/BJ20050548

Figure 4. Dose-dependent inhibition by exogenous T. spiralis His–TS (A), His-tag-free TSc (B) or His-tag-free TSt (C) of T. spiralis TS mRNA translation.

Figure 4

(A–C) Rabbit reticulocyte lysate was incubated with 6.6 pmol (0.13 μM) T. spiralis TS mRNA (lanes 1–10) and the corresponding TS preparations at concentrations of 0.6 μM (lane 2), 1.3 μM (lane 3), 2.0 μM (lane 4), 2.6 μM (lane 5) and 3.3 μM (lane 6), 3.9 μM (lane 7), 5.2 μM (lane 8), 6.6 μM (lane 9) and 7.8 μM (lane 10). (D) Results of autoradiography assessed by densitometry, expressed in arbitrary units and presented as means±% difference (bars) between the mean and each of the two results. HisTag-TS, His–TS; HisTag-free TS, His-tag-free TS.