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. 2005 Sep 5;390(Pt 3):681–688. doi: 10.1042/BJ20050548

Figure 5. The effect of exogenous DHFR, BSA, T. spiralis His–TS, rat His–TS and E. coli TS on in vitro translation of T. spiralis TS and Coleoptera luciferase mRNA.

Figure 5

Translation reaction mixtures contained no exogenous mRNA (lane 1), 6.6 pmol (0.13 μM) T. spiralis mRNA (lanes 2–10) and 3.4 pmol (34 nM) luciferase mRNA (lanes 5–10). Exogenously added proteins were 2.6 μM DHFR (lanes 3 and 9), 33 μM BSA (lanes 4 and 10), 3.3 μM T. spiralis His–TS (lane 6), 2.6 μM rat His–TS (lane 7) and 1.3 μM E. coli TS (lane 8).