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. 2005 Sep 5;390(Pt 3):681–688. doi: 10.1042/BJ20050548

Figure 6. Influences of dUMP, meTHF and FdUMP on TS protein-inhibited in vitro translation of T. spiralis TS mRNA.

Figure 6

(A) Inability of substrates to release enzyme protein-inhibited translation. Each substrate, dUMP (2.5 mM; lanes 3 and 6) or meTHF (600 μM; lanes 4 and 7), was added to the Rabbit Reticulocyte Lysate System containing 6.6 pmol (0.13 μM) T. spiralis TS mRNA (lanes 2–7) with 3.3 μM T. spiralis His–TS (HisTag–TS; lanes 5–7) or without it (lanes 2–4). (B) Enhancement of partial inhibition of translation by His–TS, His-tag-free (HisTag-free) TSc or His-tag-free TSt in the presence of FdUMP or meTHF. FdUMP (50 μM; lanes 4, 7 and 11), dUMP (2.5 mM; lanes 8 and 12) and meTHF (300 μM or 600 μM; lanes 5, 9 and 13 respectively) were added to reaction mixtures containing 6.6 pmol (0.13 μM) T. spiralis TS mRNA (lanes 2–13) and 3.3 μM T. spiralis His–TS (lanes 3–5), His-tag-free TSc (lanes 6–9) or His-tag-free TSt (lanes 10–13).