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Primer sequences and amplified product sizes
| Cytokine or protein | Type of primer | Sequence (5′ to 3′) | Amplified band size (bp) |
|---|---|---|---|
| INF-γ | Sense | GGCTGTTTCTGGCTGTTACTGC | 426 |
| Antisense | GACTCCTTTTCCGCTTCCTGA | ||
| TNF-α | Sense | CCTCCCTCTCATCAGTTCTA | 501 |
| Antisense | GCAATGACTCTAAAGTAGACCTG | ||
| IL-4 | Sense | GACGGCACAGAGCTATTGATGGGTC | 411 |
| Antisense | TAGGCTTTCCAGGAAGTCTTTCAGTGATGTG | ||
| GAPDH | Sense | TGGCCAAGGTCATCCATGAC | 210 |
| Antisense | AGGCCATGCCAGTGAGCTTC |
