TABLE 1.
Characterization of IFN-γ-producing splenic cell populations after LPS challengea
| Cell type | % of cells producing IFN-γ at the following times (h) after LPS challenge:
|
% of all cells producing IFN-γ at peak | |||
|---|---|---|---|---|---|
| 0 | 4 | 8 | 16 | ||
| DX5+ | 1.6 | 15.3 | 72.4 | 30.9 | 59.8 |
| CD3+ | 0.4 | 1.1 | 2.0 | 1.8 | 24.1 |
| CD11b+ | 0.8 | 5.8 | 9.4 | 6.0 | 4.9 |
| CD11c+ | 5.8 | 7.5 | 12.7 | 17.5 | 3.2 |
| CD19+ | 0.5 | 0.6 | 0.4 | 0.3 | 0.1 |
a
Splenocytes were isolated and stained for the individual surface marker followed by intracellular staining with anti-IFN-γ. Data are expressed as the percentage of each cell population that was IFN-γ positive and the percentage of each cell type that contributed to total IFN-γ production.