Table 3.
Standard conditions for the PCR amplification reactions
| Step | Temperature | Time | Cycle |
|---|---|---|---|
| Initial denaturation | 95 ℃ | 10 min | 1 |
| Denaturation | 95 ℃ | 10 s | 40 |
| Primer annealing and extension | 60 ℃ | 30 s | |
| Melting stage | 95 ℃ | 15 s | 1 |
| 60 ℃ | 60 s | ||
| 95 ℃ | 15 s |
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