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. Author manuscript; available in PMC: 2025 Apr 15.
Published in final edited form as: J Neurovirol. 2024 Oct 4;30(5-6):513–523. doi: 10.1007/s13365-024-01229-4

Fig. 4.

Fig. 4

Effect of latency establishment and TNF-α treatment on the expression of different sodium channel Nav transcripts as assessed by real-time PCR analysis. (A-F) Effect of ACV, ACV + TNF-α, latency establishment, and latency establishment with TNF-α treatment on the transcript expression of the Nav1.1 (A), Nav1.2 (B), Nav1.3 (C), Nav1.6 (D), and Nav1.7 (E) channel subunits. In ACV-treated cells, exposure of differentiated ND7/23 cells to TNF-α evoked a significant reduction in the expression of Nav1.3 and Nav1.7 channel transcripts (* denotes p < 0.05 vs. ACV-treated cells). Latency establishment (following ACV pre-treatment and M-GFP infection) resulted in a reduction in Nav1.7 transcripts compared to ACV-treated cells. Latency establishment followed by TNF-α treatment evokes a significant increase in the expression of the Nav1.1, Nav1.2, and Nav1.6 mRNA compared with latently infected cells. (* denotes p < 0.05 vs. M-GFP + ACV treated cells)