Fig. 6.

Effect of TNF-α signaling on the functional expression of Na⁺ channels in differentiated ND7/23 cells following latency establishment. (A) TNF-α stimulation of differentiated ND7/23 cells following ACV treatment or latency establishment does not affect cell capacitance. The p38 inhibitors SB203580 (10 μM) and SB202190 (10 μM) evoked no significant changes in cell capacitance under any treatment conditions. (B) Latency establishment (ACV + M-GFP) causes a significant reduction in the Na⁺ current densities compared to ACV-treated cells (* denotes p < 0.05 vs. ACV-treated cells). TNF-α stimulation of differentiated ND7/23 cells following latency establishment (ACV + M-GFP) evoked a significant increase in Na⁺ current densities compared to ACV + M-GFP-treated cells (** denotes p < 0.05 vs. ACV + M-GFP-treated cells). However, the stimulatory effect of TNF-α on Na⁺ channels during latency establishment was eliminated following treatment with SB203580 or SB202190 (*** denotes p < 0.05 vs. ACV + M-GFP + TNF-α treated cells)